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mouse anti isl1 2 antibody  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank mouse anti isl1 2 antibody
    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
    Mouse Anti Isl1 2 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+isl1/anti-Islet-1+%26+Islet-2+homeobox/bio_rxiv__64898__2026__05__07__723626-324-50-54
    Average 96 stars, based on 279 article reviews
    mouse anti isl1 2 antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Engineering a pacemaker-driven human mini-heart guided by spatial and single cell multi-omics of sinoatrial node development"

    Article Title: Engineering a pacemaker-driven human mini-heart guided by spatial and single cell multi-omics of sinoatrial node development

    Journal: bioRxiv

    doi: 10.64898/2026.05.07.723626

    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
    Figure Legend Snippet: a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Techniques Used: Fluorescence, Control, Staining

    Related Articles

    Purification:

    Article Title: Neural crest cell-derived DKK1 and NEDD4 modulate Wnt signalling in the second heart field to orchestrate outflow tract development
    Article Snippet: For immunolabelling, cryosections, whole embryos, or fixed cells were blocked in 10% DAKO block, 0.2% BSA, 0.2% Triton X-100 in PBS, and stained with the indicated primary antibodies. .. Antibodies used were rabbit anti-NEDD4 (Abcam 14592) 1:300; rabbit anti-NEDD4 (purified serum, gift from S. Kumar) 1:300; mouse anti-alpha smooth muscle actin (Sigma A2547) 1:2000; rat anti-CD31 (Biolegend 102502) 1:150; goat anti-SOX10 (R&D Systems AF2864) 1:200; chicken anti-GFP (Abcam ab13970) 1:1000; mouse anti-Isl1 (DSHB 39.4D5 or 40.3A4) 1:50; mouse anti-MF20 (DSHB) 1:100; goat anti-DKK1 (R&D Systems AF1765) 1:200; mouse anti-AP2a (DSHB 3B5) 1:20; rabbit anti-beta-catenin non-pS45 (Cell Signaling Technology 19807) 1:200; mouse anti-beta-catenin ABC (8E7) (Millipore 05-665) 1:100; mouse anti-beta-catenin pY489 (DSHB) 1:50; mouse anti-FLAG (Sigma F3165) 1:1000; mouse anti-GM130 (BD 610822) 1:100; rabbit anti-phospho-Histone H3 (Millipore 06-570) 1:500; rabbit anti-cleaved-Caspase-3 (Cell Signaling Technology 9661) 1:500; rabbit anti-phospho-SMAD1/5/9 (Cell Signaling Technology 13820) 1:200; rabbit anti-phospho-ERK1/2 (Cell Signaling Technology 4370) 1:100; goat anti-Scribble (Santa Cruz Biotechnology sc-11048) 1:50; rabbit anti-Laminin (Sigma L9393) 1:1000; rabbit anti-Fibronectin (DakoCytomation A0245) 1:1000; mouse anti-N-cadherin (Cell Signaling Technology 14215) 1:100; AlexaFluor 647 conjugated Phalloidin (Invitrogen) 1:200. .. EdU staining was performed following manufacturers recommendations (Invitrogen Click-iT EdU AlexaFluor 555 Cell Proliferation Kit).

    Article Title: Neural crest cell-derived DKK1 and NEDD4 modulate Wnt signalling in the second heart field to orchestrate outflow tract development.
    Article Snippet: For immunolabelling, cryosections, whole embryos or fixed cells were blocked in 10% DAKO block, 0.2% BSA, 0.2% Triton X-100 in PBS, and stained with the indicated primary antibodies. .. Antibodies used were rabbit anti-NEDD4 (Abcam 14592) 1:300; rabbit anti-NEDD4 (purified serum, gift from S. Kumar) 1:300; mouse anti-alpha smooth muscle actin (Sigma A2547) 1:2000; rat anti-CD31 (Biolegend 102502) 1:150; goat anti-SOX10 (R&D Systems AF2864) 1:200; chicken anti-GFP (Abcam ab13970) 1:1000; mouse anti-Isl1 (DSHB 39.4D5 or 40.3A4) 1:50; mouse anti-MF20 (DSHB) 1:100; goat anti-DKK1 (R&D Systems AF1765) 1:200; mouse anti-AP2a (DSHB 3B5) 1:20; rabbit anti-beta-catenin non-pS45 (Cell Signaling Technology 19807) 1:200; mouse anti-beta-catenin ABC (8E7) (Millipore 05-665) 1:100; mouse anti-beta-catenin pY489 (DSHB) 1:50; mouse antiFLAG (Sigma F3165) 1:1000; mouse anti-GM130 (BD 610822) 1:100; rabbit antiphospho-Histone H3 (Millipore 06-570) 1:500; rabbit anti-cleaved-Caspase-3 (Cell AR TI CL E IN P RE SS Signaling Technology 9661) 1:500; rabbit anti-phospho-SMAD1/5/9 (Cell Signaling Technology 13820) 1:200; rabbit anti-phospho-ERK1/2 (Cell Signaling Technology 4370) 1:100; goat anti-Scribble (Santa Cruz Biotechnology sc-11048) 1:50; rabbit antiLaminin (Sigma L9393) 1:1000; rabbit anti-Fibronectin (DakoCytomation A0245) 1:1000; mouse anti-N-cadherin (Cell Signaling Technology 14215) 1:100; Alexa Fluor 647 conjugated Phalloidin (Invitrogen) 1:200. .. EdU staining was performed following manufacturers recommendations (Invitrogen Click-iT EdU Alexa Fluor 555 Cell Proliferation Kit).

    Labeling:

    Article Title: Convergent flow-mediated mesenchymal force drives embryonic foregut constriction and splitting
    Article Snippet: After three washes with the Washing Buffer, the samples were mounted in ProLong Diamond Antifade Mountant (Invitrogen, P36970 ) with #1 coverglass (VWR, 48393-106). .. Primary antibodies used were: rat anti-SOX2 (1:300, Invitrogen, 14-9811-82), rabbit anti-NKX2-1 (1:300, Abcam, 76013), rabbit anti-cleaved Caspase 3 (1:300, Cell Signaling Technology, 9661), rabbit anti-phospho-Histone H3 (1:300, Cell Signaling Technology, 3377), rabbit anti phospho-MLC2 (1:100, Cell Signaling Technology, 3674), rabbit anti-mCherry (1:500, Abcam, 167453, for labeling tdTomato), biotinylated Hyaluronic Acid Binding Protein (1:200, Sigma-Aldrich, 385911), mouse anti-GM130 (1:150, BD Biosciences, 610822), rabbit anti-phospho-FAK (1:200, Invitrogen, 700255), goat anti-NKX6-1 (10 μg/mL, R&D Systems, AF5857), rabbit anti-TBX1 (1:50, Invitrogen, 34-9800), mouse anti-ISL1 (1:30, DSHB, 40.2D6), mouse anti-ALDH1A2 (1:50, Santa Cruz Biotechnology, sc-393204), goat anti-SHH (1:100, R&D Systems, AF464). .. Dye-conjugated secondary antibodies were from Jackson ImmunoResearch (with Alexa Fluor 647, Cy3, or Alexa Fluor 488) and used at 1:300 dilution, except the streptavidin-Alexa Fluor 647 for HABP detection (1:300, Invitrogen, S32357 ).

    Binding Assay:

    Article Title: Convergent flow-mediated mesenchymal force drives embryonic foregut constriction and splitting
    Article Snippet: After three washes with the Washing Buffer, the samples were mounted in ProLong Diamond Antifade Mountant (Invitrogen, P36970 ) with #1 coverglass (VWR, 48393-106). .. Primary antibodies used were: rat anti-SOX2 (1:300, Invitrogen, 14-9811-82), rabbit anti-NKX2-1 (1:300, Abcam, 76013), rabbit anti-cleaved Caspase 3 (1:300, Cell Signaling Technology, 9661), rabbit anti-phospho-Histone H3 (1:300, Cell Signaling Technology, 3377), rabbit anti phospho-MLC2 (1:100, Cell Signaling Technology, 3674), rabbit anti-mCherry (1:500, Abcam, 167453, for labeling tdTomato), biotinylated Hyaluronic Acid Binding Protein (1:200, Sigma-Aldrich, 385911), mouse anti-GM130 (1:150, BD Biosciences, 610822), rabbit anti-phospho-FAK (1:200, Invitrogen, 700255), goat anti-NKX6-1 (10 μg/mL, R&D Systems, AF5857), rabbit anti-TBX1 (1:50, Invitrogen, 34-9800), mouse anti-ISL1 (1:30, DSHB, 40.2D6), mouse anti-ALDH1A2 (1:50, Santa Cruz Biotechnology, sc-393204), goat anti-SHH (1:100, R&D Systems, AF464). .. Dye-conjugated secondary antibodies were from Jackson ImmunoResearch (with Alexa Fluor 647, Cy3, or Alexa Fluor 488) and used at 1:300 dilution, except the streptavidin-Alexa Fluor 647 for HABP detection (1:300, Invitrogen, S32357 ).



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    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
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    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number <t>of</t> <t>ISL1/2</t> + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.
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    Image Search Results


    a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Journal: bioRxiv

    Article Title: Engineering a pacemaker-driven human mini-heart guided by spatial and single cell multi-omics of sinoatrial node development

    doi: 10.64898/2026.05.07.723626

    Figure Lengend Snippet: a, Schematic of zebrafish drug treatment experiments. b, Representative bright-field images of zebrafish larvae at 54 hpf. Scale bar=100 µm. c, Representative fluorescence images of zebrafish /larvae at 54 hpf treated with 5 µM Afatinib, 5 uM BAY-593 (YAPi) or vehicle control. Scale bar=100 µm. d, e, Quantification of the number of ISL1/2 + cells (c) and ISL1/2 + area in zebrafish larvae treated with 5 uM Afatinib, 5 uM BAY-593 or vehicle control. Data are shown as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each experiment, N = 25 larvae. For each drug-treated set there was a consistent loss of Isl1-staining; 3 larvae from each set were randomly chosen for quantification, carried out by a blinded investigator.

    Article Snippet: At 54 hpf larvae were fixed in 2% PFA for 2 hours at room temperature, washed extensively in PBS with 0.3% TritonX-100 (PBX), incubated in blocking buffer (BB: PBX, 0.5% BSA, 10% goat serum) for 2 hours at room temperature, incubated overnight at 4°C in BB containing 1/50 dilution of mouse anti-ISL1/2 antibody (Iowa Developmental Studies Hybridoma Bank #39.4D5), washed extensively with PBX, incubated at room temperature for 2 hours in BB with a 1/200 dilution of goat anti-mouse IgG2b Alexa Fluor568 (Invitrogen #A-21144), washed extensively with PBX, and mounted in 1:1 PBS:glycerol for imaging on a Zeiss LSM800 confocal microscope.

    Techniques: Fluorescence, Control, Staining